Coding

Part:BBa_K4102000:Design

Designed by: Friedrich J. Ehinger   Group: iGEM21_Bonn-Rheinbach   (2021-10-07)


lanM gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 109
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The translocation tag had to be excluded to prevent translocation to the peri- or exoplasm and loss to the supernatant. High GC content at the 5' end was not problematic.


Source

The gene was amplified from the natural host Methylorubrum extorquens PA1 and the hexa-His-tag was incorporated through primer design.

References